Cell Segmentation with Globally Optimized boundaries (CSGO) is a whole-cell segmentation pipeline tailored specifically to the H&E domain. CSGO brings four notable contributions:

1) a U-Net algorithm to detect cell membranes with various intensities; 2) integration of HD-Yolo nuclei detection with the U-Net membrane detection; 3) superior performance and robustness: CSGO consistently outperforms the state-of-the-art cell segmentation algorithm across a range of cancer types; all while requiring fewer manual annotations; and 4) this web-based software to facilitate CSGO usage.