What is multiTAP?
Multiplexed Tissue Analysis Platform (MultiTAP) is an open-source Python package designed for analyzing multiplexed tissue images. Package functionalities include the quantification of single-cell biomarkers, cell type clustering, and the analysis of cell-cell interactions. MultiTAP addresses a critical gap between the existing software landscape and the exponentially increasing volume of multiplexed tissue image data, by providing comprehensive and user-friendly tools tailored specifically for analyzing multiplexed data representations. Compared with previous software, MultiTAP offers unique features and advantages. It incorporates a cell segmentation algorithm guided by cytoplasm membrane labels, provides downstream analysis methods such as data normalization and automatic identification of marker-positive cells, and enables co-expression and cell-cell spatial interaction analysis. Moreover, MultiTAP offers the convenience of end-to-end analysis with a GUI for users from diverse backgrounds.